One or more protein sequences can be input into the server in FASTA format. The server accepts at most 200 protein sequences per job. The user can either paste one or more sequences in the text-area provided, or, alternatively, upload a FASTA file containing protein sequences.
The BUSCA home page looks like the following:
Besides the actual input sequences, the user must specify (through the designated selection menu) which organism class the sequences belong to, chosing among:
Example input sequences can be automatically loaded into the text-area using the "Example FASTA" button. To submit the job, the user should simply click on the "Start prediction" button.
Upon submission, the user will be automatically redirected to the page where the job results will be available after completion. If the job is still running, the result table shows no content.
The typical BUSCA output looks like the following:
In the first section, main information about the job is reported.
Job information include:
The section "Predicted GO-terms and protein featues" contains the actual prediction result, displayed as a paginated table. By default, 10 entries are shown on each page. Clicking on the pagination buttons, the user can browse results. For each submitted protein, the following data are reported:
By clicking on the expand details button (marked with the icon and placed on the left of each protein accession), it is possible to visualize features predicted on that specific protein, if any. Different features are visualized on different annotation tracks along the sequence:
The user can zoom in by left-clicking and selecting the area of interest:
This allows to see feature annotation at residue-level:
To zoom out just right-click on the feature track to reset the scale. Note that, sometimes, when the sequence is too long (as in the example shown), the actual residues are not visible at the initial default zoom level.
The feature panel can be closed by clicking on . The button "Show help" provides an explaination on how to use the interactive feature panel.
The "Search" box can be used to free-text search the result table, displaying only entries meeting provided search criteria. Furthermore, result table can be sorted by any field by clicking on the corresponding field header.
Using the buttons "Download JSON" and "Download CSV" the user can download the job results in either JSON or CSV formats.