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BUSCA (Bologna Unified Subcellular Component Annotator) is a web-server for predicting protein subcellular localization. BUSCA integrates different tools to predict localization-related protein features (DeepSig, TPpred3, PredGPI, BetAware and ENSEMBLE3.0) as well as tools for discriminating subcellular localization of both globular and membrane proteins (BaCelLo, MemLoci and SChloro).

For details see: Savojardo, C, Martelli, PL, Fariselli, P, Profiti, G, Casadio, R (2018) BUSCA: an integrative web server to predict subcellular localization of proteins. Nucleid Acids Research, 46(W1), W459-W466.

Prediction of subcellular localization

Prediction of protein features

Tools in BUSCA

BUSCA is able to predict:

  • 16 compartments for plant proteins
  • 9 compartments for animal or fungi proteins
  • 4 compartments for Gram-negative bacterial proteins
  • 3 compartments for Gram-positive bacterial proteins

BUSCA provides residue-level annotation of the following features:

  • Secretory signal peptides
  • Organelle-targeting peptides (mitochondrial and chloroplastic)
  • GPI-anchors
  • Alpha and beta transmembrane regions

DeepSig TPpred3 PredGPI
BaCelLo SChloro BetAware
MemLoci ENSEMBLE3.0  

Submit your sequence(s)

Please, paste input FASTA sequences (max 500 sequences, max 400000 residues) in the area below:

Alternatively, select and upload a FASTA file:

Select the taxonomic origin of the input sequences: